Composite

Part:BBa_K823050:Design

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-09-10)

luc luciferase, codon optimized for Bacillus subtilis+ RBS+Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 625
    Illegal SapI site found at 853


Design Notes

only 1 3A-assembly needed to combine it with the desired promoter


Source

luc: synthesized by gene art

B0014: registry

References

Reference:[http://www.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/bioluminescent-reporters/ Promega]